NanoSight ND filters

You may have observed in analysing biological samples on your NanoSight , that very large particles present with your nano particles are hard to image.

Being large they flare strongly, move slowly, dominate  and upset the NTA tracking analysis.

"Neutral density filter demonstration" by Robert Emperley from Strasbourg, Alsace, France - silver falls 21Uploaded by NotFromUtrecht. Licensed under CC BY-SA 2.0 via Wikimedia Commons.

“Neutral density filter demonstration” by Robert Emperley from Strasbourg, Alsace, France – silver falls 21Uploaded by NotFromUtrecht. Licensed under CC BY-SA 2.0 via Wikimedia Commons.

This of course can be  avoided by filtering out the particles in the >400nm regions; but you may not want to do that analytically. Nor may you wish to run sequential measures.

An alternative is to fit a Neutral Density filter on the filter wheel and measure with the ND filter in place, this reduces the strong VLP scattering and enables the sensitive camera to see and size the nano materials at the same time. An example is of this mix of latex standards run with ND1 in place (Roberto Ghiandoni).

If you wish to purchase one of these for your system, the description is OPF0059 Neutral Density Filter ND1.0 and yes, we are fitting one to our demonstration system. With NS300 and NS500 systems fitting to the wheel is an FSE task we can do FOC. Use in an LM10 is a manual drop in by the user.

 

 

 

 

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